Article
Cloning and characterization of an iron regulated locus, iroA, in Salmonella enterica serovar Choleraesuis.
DNA sequence : the journal of DNA sequencing and mapping - 1 Dec 2002
Wu Wen-Sheng, Hsieh Pei-Chen, Huang Tzu-Min, Chang Yung-Fu, Chang Chao-Fu
Abstract excerpt
To identify genes belonging to the Ferric update regulator (Fur) regulon of Salmonella enterica serovar Choleraesuis, the Fur titration assay (FURTA) was used to screen a genomic library for Fur promoters and iron-regulated genes. Fifteen FURTA positive clones were identified from this assay. DNA sequence analysis of these clones showed that 11 out of 15 clones had a Fur binding site (Fur box), and 6 of these...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Iron
- Mutation
- Peptide Library
- Promoter Regions, Genetic
- Salmonella enterica
- Sequence Analysis, DNA
