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Article

The spoilage yeast Zygosaccharomyces bailii forms mitotic spores: a screening method for haploidization.

Applied and environmental microbiology - 1 Jan 2003

Rodrigues Fernando, Ludovico Paula, Sousa Maria João, Steensma H Yde, Côrte-Real Manuela, Leão Cecília

Abstract excerpt

Zygosaccharomyces bailii ISA 1307 and the type strain of this spoilage yeast show a diploid DNA content. Together with a rather peculiar life cycle in which mitotic but no meiotic spores appear to be formed, the diploid DNA content explains the observed difficulties in obtaining auxotrophic mutants. Mitotic chromosome loss induced by benomyl and selection on canavanine media resulted in three haploid strains of...

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Identifiers

PMID: 12514054
DOI: 10.1128/AEM.69.1.649-653.2003
PMCID: PMC152455

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