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Article

Isolation of mutants and construction of intersexual heterokaryons of Blakeslea trispora.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2012

Cerdá-Olmedo Enrique, Mehta Bina J

Abstract excerpt

The Mucoral fungus Blakeslea trispora is used for the industrial production of β-carotene and lycopene. Two genetic techniques have been used to increase carotene accumulation: the isolation of mutants and the formation and segregation of heterokaryons. Because all life stages are multinucleated, recessive mutants are isolated after exposure to N-methyl-N'-nitro-N-nitrosoguanidine, a strong mutagen and...

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PMID: 22711118
DOI: 10.1007/978-1-61779-918-1_4

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