Article

Estimating the true stability of the prehydrolytic outward-facing state in an ABC protein

2023-07-18

Abstract excerpt

CFTR, the anion channel mutated in cystic fibrosis patients, is a model ABC protein whose ATP-driven conformational cycle is observable at single-molecule level in patch-clamp recordings. Bursts of CFTR pore openings are coupled to tight dimerization of its two nucleotide binding domains (NBDs) and in wild-type (WT) channels are mostly terminated by ATP hydrolysis. The slow rate of non-hydrolytic closure – which d...

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Literature Corpus work: fb359641-19a5-5826-8d4c-bb44c5d952a5
DOI: 10.1101/2023.07.16.549217

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