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Article

Directed evolution of substrate-optimized GroEL/S chaperonins.

Cell - 27 Dec 2002

Wang Jue D, Herman Christophe, Tipton Kimberly A, Gross Carol A, Weissman Jonathan S

Abstract excerpt

GroEL/S chaperonin ring complexes fold many unrelated proteins. To understand the basis and extent of the chaperonin substrate spectrum, we used rounds of selection and DNA shuffling to obtain GroEL/S variants that dramatically enhanced folding of a single substrate-green fluorescent protein (GFP). Changes in the substrate-optimized chaperonins increase the polarity of the folding cavity and alter the ATPase...

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Identifiers

PMID: 12507429
DOI: 10.1016/s0092-8674(02)01198-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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