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Article

One step purification of alpha(1)-acid glycoprotein from human plasma. Fractionation of its polymorphic allele products.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 25 Jan 2003

Azzimonti Federica, Atchley Daniel H, Morrison Catherine Anne, Dodd Seetal, Boulton David W, DeVane C Lindsay, Arnaud Philippe

Abstract excerpt

Alpha(1)-acid glycoprotein is a plasma protein that exhibits both microheterogeneity and polymorphism. Its purification from human plasma is usually performed using a sequence of different fractionation steps. Here we report a one-step isolation technique of this protein based upon pseudo-ligand affinity chromatography on immobilized Cibacron Blue F3GA at acidic pH. In addition, the use of two narrow pH elution...

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PMID: 12504180
DOI: 10.1016/s1570-0232(02)00749-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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