Article
Purification of human haptoglobin 1-1, 2-1, and 2-2 using monoclonal antibody affinity chromatography.
Protein expression and purification - 1 Feb 2004
Tseng Chi Feng, Huang Hsing Yi, Yang Yuan Ting, Mao Simon J T
Abstract excerpt
Similar to blood type, human plasma haptoglobin (Hp) is classified as 3 phenotypes: Hp 1-1, 2-1, or 2-2. The structural and functional relationship between the phenotypes, however, has not been studied in detail due to the complicated and difficult isolation procedures. This report provides a simple protocol that can be used to purify each Hp phenotype. Plasma was first passed through an affinity column coupled...
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