Article
High-performance anion-exchange chromatographic study of desialylated human alpha 1-acid glycoprotein variants. Development of a fractionation method for the protein slow variants.
Journal of chromatography - 22 Feb 1991
Herve F, Duche J C, Sportes N, Tillement J P
Abstract excerpt
The three main desialylated variants (F1, S and A) of human alpha 1-acid glycoprotein (AAG), a serum acute-phase reactant, were analysed by high-performance anion-exchange chromatography in order to determine their optimum separation conditions. The analysis consisted of three steps, as follows: (1) A desialylated commercial AAG was separated into one "fast"- and one "slow"-migrating fraction by preparative...
Topics
- Chromatography, Ion Exchange
- Genetic Variation
- Humans
- Hydrogen-Ion Concentration
- Orosomucoid
- Phenotype
- Protein Synthesis Inhibitors
- Triazines