Article
Studies of cargo delivery to the vacuole mediated by autophagosomes in Saccharomyces cerevisiae.
Developmental cell - 1 Dec 2002
Suzuki Kuninori, Kamada Yoshiaki, Ohsumi Yoshinori
Abstract excerpt
In the yeast Saccharomyces cerevisiae, aminopeptidase I (API), a vacuolar hydrolase, is selectively transported to the vacuole via the autophagosome. API forms a cytosol to vacuole targeting (Cvt) complex in the cytoplasm. The complex is engulfed by the autophagosome under starvation conditions. In this study, the Cvt complex is visualized as a dot in the cytoplasm by fluorescence microscopy with API-GFP. The Cvt...
Topics
- Aminopeptidases
- Autophagy
- Autophagy-Related Proteins
- Cytosol
- Food Deprivation
- Green Fluorescent Proteins
- Luminescent Proteins
- Microscopy, Fluorescence
- Mutation
- Phagosomes
- Protein Transport
- Receptors, Cell Surface
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Signal Transduction
- Sirolimus
- Transport Vesicles