Article
The TAR RNA-binding protein, TRBP, stimulates the expression of TAR-containing RNAs in vitro and in vivo independently of its ability to inhibit the dsRNA-dependent kinase PKR.
The Journal of biological chemistry - 14 Feb 2003
Dorin Dominique, Bonnet Marion C, Bannwarth Sylvie, Gatignol Anne, Meurs Eliane F, Vaquero Catherine
Abstract excerpt
TRBP (HIV-1 transactivating response (TAR) RNA-binding protein) and PKR, the interferon-induced dsRNA-regulated protein kinase, contain two dsRNA binding domains. They both bind to HIV-1 TAR RNAs through different sites. Binding to dsRNA activates PKR that phosphorylates the eukaryotic initiation factor eIF-2alpha leading to protein synthesis inhibition. TRBP and PKR can heterodimerize, which inhibits the kinase...
Topics
- Animals
- Cells, Cultured
- Genes, Reporter
- Mice
- Mutation
- Phosphorylation
- Protein Biosynthesis
- Protein Structure, Tertiary
- RNA
- RNA-Binding Proteins
- Signal Transduction
- Transcription, Genetic
- eIF-2 Kinase