Article
Using satellite tobacco mosaic virus vectors for gene silencing.
Current protocols in microbiology - 1 Oct 2005
Gosselé Véronique, Metzlaff Michael
Abstract excerpt
This unit describes the use of satellite tobacco mosaic virus (STMV) vectors in combination with native TMV particles for inducing transient gene silencing in tobacco plants. Target gene fragment selection and insertion, virus delivery procedures, and phenotype screening of silenced plants are described in detail. All critical parameters for tobacco plant cultivation, virus infection, and RNA silencing efficiency...
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