Article
Definitive high resolution typing of HLA-E allelic polymorphisms: Identifying potential errors in existing allele data.
Tissue antigens - 1 Sept 2002
Grimsley C, Kawasaki A, Gassner C, Sageshima N, Nose Y, Hatake K, Geraghty D E, Ishitani A
Abstract excerpt
A set of robust PCR-SSP reactions were developed for each of the five polymorphic sites that define the five alleles of the HLA class Ib gene, HLA-E. This method was developed using 28 homozygous cell lines and further tested in a sample of African-Americans, a sample of Japanese, and a core pane...
Topics
- Alleles
- HLA Antigens
- Histocompatibility Antigens Class I
- Histocompatibility Testing
- Humans
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Reproducibility of Results
- Sequence Analysis, DNA
- HLA-E Antigens