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Article

Development of a positive genetic selection system for inhibition of protein splicing using mycobacterial inteins in Escherichia coli DNA gyrase subunit A.

Journal of molecular microbiology and biotechnology - 1 Sept 2002

Adam Eric, Perler Francine B

Abstract excerpt

An intein-based positive genetic selection system was developed to study protein splicing and to provide a selection system with the potential for finding splicing inhibitors. Inteins can be novel antimicrobial targets when present in essential proteins since blocking splicing would kill the organism. For example, pathogenic mycobacteria encode inteins that interrupt DNA gyrase. The gyrase selection system...

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PMID: 12432958

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Development of a positive genetic selection system for inhibition of protein splicing using mycobacterial inteins in Escherichia coli DNA gyrase subunit A.PMID 12432958Read ↗

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Temperature-sensitive mutations made easy: generating conditional mutations by using temperature-sensitive inteins that function within different temperature ranges.Semantic proximity Read ↗

Probing intein-catalyzed thioester formation by unnatural amino acid substitutions in the active site.Semantic proximity Read ↗

Post-translational environmental switch of RadA activity by extein-intein interactions in protein splicing.Semantic proximity Read ↗

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