Article
Identification of temperature-sensitive mutations in the phosphoprotein of respiratory syncytial virus that are likely involved in its interaction with the nucleoprotein.
Journal of virology - 1 Mar 2002
Lu Bin, Brazas Robert, Ma Chien-Hui, Kristoff Tina, Cheng Xing, Jin Hong
Abstract excerpt
The phosphoprotein (P) of human respiratory syncytial virus (RSV) is an essential component of the viral RNA polymerase, along with the large polymerase (L), nucleocapsid (N), and M2-1 proteins. By screening a randomly mutagenized P gene cDNA library, two independent mutations, one with a substit...
Topics
- Animals
- Cell Line
- Chlorocebus aethiops
- Humans
- Mice
- Mutation
- Nucleoproteins
- Phosphoproteins
- Rats
- Recombination, Genetic
- Respiratory Syncytial Virus Infections
- Respiratory Syncytial Virus, Human
- Temperature
- Two-Hybrid System Techniques
- Vero Cells