Article
Genotypic and phenotypic characterization of the thymidine kinase of ACV-resistant HSV-1 derived from an acyclovir-sensitive herpes simplex virus type 1 strain.
Antiviral research - 1 Dec 2002
Saijo Masayuki, Suzutani Tatsuo, De Clercq Erik, Niikura Masahiro, Maeda Akihiko, Morikawa Shigeru, Kurane Ichiro
Abstract excerpt
Twenty-four strains of acyclovir (ACV)-resistant (ACV(r)) herpes simplex virus type 1 (HSV-1) were generated from the HSV-1 TAS strain by exposure to ACV, and the genotype and phenotype of the thymidine kinase (TK) from these mutants were analyzed. The TK polypeptide of the ACV(r) HSV-1 strains was examined by Western blot using an anti-HSV-1 TK rabbit serum. The sensitivity of each strain to ACV, foscarnet and...
Topics
- Acyclovir
- Amino Acid Sequence
- Animals
- Antiviral Agents
- Base Sequence
- Blotting, Western
- Chlorocebus aethiops
- Cidofovir
- Cytosine
- Drug Resistance, Viral