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Article

Purification and characterization of Snf1 kinase complexes containing a defined Beta subunit composition.

The Journal of biological chemistry - 27 Dec 2002

Nath Nandita, McCartney Rhonda R, Schmidt Martin C

Abstract excerpt

The Snf1 kinase complex of Saccharomyces cerevisiae contains one of three possible beta subunits encoded by either SIP1, SIP2, or GAL83. Snf1 kinase complexes were purified from cells expressing only one of the three beta subunits using a tandem affinity purification tag on the C terminus of the Snf1 protein. The purified kinase complexes were enzymatically active as judged by their ability to phosphorylate a...

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PMID: 12393914
DOI: 10.1074/jbc.M207058200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Purification and characterization of Snf1 kinase complexes containing a defined Beta subunit composition.PMID 12393914Read ↗

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