Article
Nucleobase amino acids incorporated into the HIV-1 nucleocapsid protein increased the binding affinity and specificity for a hairpin RNA.
Chembiochem : a European journal of chemical biology - 3 Jun 2002
Takahashi Tsuyoshi, Ueno Akihiko, Mihara Hisakazu
Abstract excerpt
L-alpha-amino acids with a nucleobase in the side chain (nucleobase amino acids; NBAs) were used to enhance the function of RNA-binding proteins that recognize structured RNA. These NBAs were utilized in the three-dimensional structure of the protein to enhance RNA binding affinity and specificity as a result of selective recognition of NBAs by RNA bases. NBA units were incorporated at various positions into the...
Topics
- Amino Acid Sequence
- Amino Acids
- Base Sequence
- Fluoresceins
- Fluorescence Polarization
- HIV-1
- Humans
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Nucleocapsid Proteins
- Protein Binding