Article
Rapid detection of the CCR2-V64I, CCR5-A59029G and SDF1-G801A polymorphisms by tetra-primer PCR.
Clinical biochemistry - 1 Jul 2002
Hersberger Martin, Marti-Jaun Jacqueline, Hänseler Edgar, Speck Roberto F
Abstract excerpt
OBJECTIVE: To develop tetra-primer PCR assays for detection of the CCR2-V64I, CCR5-A59029G and SDF1-G801A polymorphisms associated with HIV pathogenesis. DESIGN AND METHODS: For each assay, two primers for the amplification of the gene locus are combined in one tube with two primers for the subsequent allele specific amplification (ASA). In the first set of cycles, pre-amplification of the gene region of interest...
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