Article
Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes.
Biotechnology and bioengineering - 5 Sept 2002
Bailey James E, Sburlati Adriana, Hatzimanikatis Vassily, Lee Kelvin, Renner Wolfgang A, Tsai Philip S
Abstract excerpt
The classical method of metabolic engineering, identifying a rate-determining step in a pathway and alleviating the bottleneck by enzyme overexpression, has motivated much research but has enjoyed only limited practical success. Intervention of other limiting steps, of counter-balancing regulation, and of unknown coupled pathways often confounds this direct approach. Here the concept of inverse metabolic...
Topics
- Animals
- Bacterial Proteins
- CHO Cells
- Cell Culture Techniques
- Cell Cycle
- Cells
- Cloning, Molecular
- Cricetinae
- Enzymes
- Genetic Engineering
- Growth Substances
- Hemoglobins
- Humans
- Mammals
- Metabolism
- Models, Biological
- Phenotype
- Recombination, Genetic