Article
Development and Evaluation of Three Real-Time PCR Assays for Genotyping and Source Tracking Cryptosporidium spp. in Water.
Applied and environmental microbiology - 1 Sept 2015
Li Na, Neumann Norman F, Ruecker Norma, Alderisio Kerri A, Sturbaum Gregory D, Villegas Eric N, Chalmers Rachel, Monis Paul, Feng Yaoyu, Xiao Lihua
Abstract excerpt
The occurrence of Cryptosporidium oocysts in drinking source water can present a serious public health risk. To rapidly and effectively assess the source and human-infective potential of Cryptosporidium oocysts in water, sensitive detection and correct identification of oocysts to the species level (genotyping) are essential. In this study, we developed three real-time PCR genotyping assays, two targeting the...
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