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Article

High resolution melting curve analysis as a new tool for rapid identification of canine parvovirus type 2 strains.

Molecular and cellular probes - 1 Jan 2000

Bingga Gali, Liu Zhicheng, Zhang Jianfeng, Zhu Yujun, Lin Lifeng, Ding Shuangyang, Guo Pengju

Abstract excerpt

A high resolution melting (HRM) curve method was developed to identify canine parvovirus type 2 (CPV-2) strains by nested PCR. Two sets of primers, CPV-426F/426R and CPV-87R/87F, were designed that amplified a 52 bp and 53 bp product from the viral VP2 capsid gene. The region amplified by CPV-426F/426R included the A4062G and T4064A mutations in CPV-2a, CPV-2b and CPV-2c. The region amplified by CPV-87F/87R...

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PMID: 25159576
DOI: 10.1016/j.mcp.2014.08.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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