Article
Real-time PCR for the detection of Cryptosporidium parvum.
Journal of microbiological methods - 1 Dec 2001
Higgins J A, Fayer R, Trout J M, Xiao L, Lal A A, Kerby S, Jenkins M C
Abstract excerpt
Real time, TaqMan PCR assays were developed for the Cp11 and 18S rRNA genes of the protozoan parasite Cryptosporidium parvum. The TaqMan probes were specific for the genus Cryptosporidium, but could not hybridize exclusively with human-infectious C. parvum species and genotypes. In conjunction with development of the TaqMan assays, two commercial kits, the Mo Bio UltraClean Soil DNA kit, and the Qiagen QIAamp DNA...
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