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Article

Evolvability of random polypeptides through functional selection within a small library.

Protein engineering - 1 Jul 2002

Yamauchi Asao, Nakashima Toshihiro, Tokuriki Nobuhiko, Hosokawa Masato, Nogami Hideki, Arioka Shingo, Urabe Itaru, Yomo Tetsuya

Abstract excerpt

A directed evolution with phage-displayed random polypeptides of about 140 amino acid residues was followed until the sixth generation under a selection based on affinity to a transition state analog for an esterase reaction. The experimental design deliberately limits the observation to only 10...

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Identifiers

PMID: 12200545
DOI: 10.1093/protein/15.7.619

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-12200545-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

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