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Article

Evolvability of random polypeptides through functional selection within a small library.

Protein engineering - 1 Jul 2002

Yamauchi Asao, Nakashima Toshihiro, Tokuriki Nobuhiko, Hosokawa Masato, Nogami Hideki, Arioka Shingo, Urabe Itaru, Yomo Tetsuya

Abstract excerpt

A directed evolution with phage-displayed random polypeptides of about 140 amino acid residues was followed until the sixth generation under a selection based on affinity to a transition state analog for an esterase reaction. The experimental design deliberately limits the observation to only 10 clones per generation. The first generation consists of three soluble random polypeptides and seven arbitrarily chosen...

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Identifiers

PMID: 12200545
DOI: 10.1093/protein/15.7.619

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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