Article
Gene disruption by homologous recombination in the Xylella fastidiosa citrus variegated chlorosis strain.
Applied and environmental microbiology - 1 Sept 2002
Gaurivaud Patrice, Souza Leonardo C A, Virgílio Andrea C D, Mariano Anelise G, Palma Renê R, Monteiro Patrícia B
Abstract excerpt
Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for beta-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to beta-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used...
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