Article
A ku70 null mutant improves gene targeting frequency in the fungal pathogen Verticillium dahliae.
World journal of microbiology & biotechnology - 1 Dec 2015
Qi Xiliang, Su Xiaofeng, Guo Huiming, Qi Juncang, Cheng Hongmei
Abstract excerpt
To overcome the challenges met with gene deletion in the plant pathogen Verticillium dahliae, a mutant strain with impaired non-homologous end joining DNA repair was generated to improve targeted gene replacement frequencies. A V. dahliae 991 ΔVdku70 null mutant strain was generated using Agrobacterium tumefaciens-mediated transformation. Despite having impaired non-homologous end joining DNA repair function, the...
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