Article
Deletion and allelic exchange of the Aspergillus fumigatus veA locus via a novel recyclable marker module.
Eukaryotic cell - 1 Jul 2005
Krappmann Sven, Bayram Ozgür, Braus Gerhard H
Abstract excerpt
Detailed evaluation of gene functions in an asexual fungus requires advanced methods of molecular biology. For the generation of targeted gene deletions in the opportunistic pathogen Aspergillus fumigatus we designed a novel blaster module allowing dominant selection of transformants due to resistance to phleomycin as well as dominant (counter)selection of a Cre recombinase-mediated marker excision event. For...
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