Article
Evidence for a novel, strongly bound acto-S1 complex carrying ADP and phosphate stabilized in the G680V mutant of Dictyostelium myosin II.
Biochemistry - 30 Jul 2002
Uyeda Taro Q P, Tokuraku Kiyotaka, Kaseda Kuniyoshi, Webb Martin R, Patterson Bruce
Abstract excerpt
Gly 680 of Dictyostelium myosin II sits at a critical position within the reactive thiol helices. We have previously shown that G680V mutant subfragment 1 largely remains in strongly actin-bound states in the presence of ATP. We speculated that acto-G680V subfragment 1 complexes accumulate in the A.M.ADP.P(i) state on the basis of the biochemical phenotypes conferred by mutations which suppress the G680V mutation...
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