Article
Carbamoyl-phosphate synthetase. Creation of an escape route for ammonia.
The Journal of biological chemistry - 18 Oct 2002
Thoden James B, Huang Xinyi, Raushel Frank M, Holden Hazel M
Abstract excerpt
Carbamoyl-phosphate synthetase catalyzes the production of carbamoyl phosphate through a reaction mechanism requiring one molecule of bicarbonate, two molecules of MgATP, and one molecule of glutamine. The enzyme from Escherichia coli is composed of two polypeptide chains. The smaller of these belongs to the Class I amidotransferase superfamily and contains all of the necessary amino acid side chains required for...
Topics
- Adenosine Triphosphate
- Ammonia
- Binding Sites
- Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor
- Crystallography, X-Ray
- Electrons
- Escherichia coli
- Histidine
- Models, Chemical
- Models, Molecular
- Mutation
- Protein Binding
- Serine
- X-Rays