Article
Transposition of pGh9:ISS1 is random and efficient in Streptococcus thermophilus CNRZ368.
Canadian journal of microbiology - 1 May 2002
Thibessard Annabelle, Fernandez Annabelle, Gintz Brigitte, Decaris Bernard, Leblond-Bourget Nathalie
Abstract excerpt
Streptococcus thermophilus bacteria are used as a starter in the fermentation of yogurts and many cheeses. To construct mutants of S. thermophilus CNRZ368, the use of the plasmid pGh9:ISS1 was considered. This plasmid is known to be a good tool for insertional mutagenesis in gram-positive bacteria, owing to its ability to integrate in the genome by a mechanism of replicative transposition. However, the presence...
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