Article
Cofactor-dependent assembly of the flavoenzyme vanillyl-alcohol oxidase.
The Journal of biological chemistry - 27 Sept 2002
Tahallah Nora, Van Den Heuvel Robert H H, Van Den Berg Willy A M, Maier Claudia S, Van Berkel Willem J H, Heck Albert J R
Abstract excerpt
The oligomerization of the flavoprotein vanillyl-alcohol oxidase (VAO) and its site-directed mutant H61T was studied by mass spectrometry. Native VAO has a covalently bound FAD and forms primarily octameric assemblies of 507 kDa. H61T is purified as a FAD-free apoprotein and mainly exists as a dimeric species of 126 kDa. Binding of FAD to apoH61T rapidly restores enzyme activity and induces octamerization,...
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