Article
Sampling tumor-draining lymph nodes for phenotypic and functional analysis of dendritic cells and T cells.
The American journal of pathology - 1 Jul 2002
Vuylsteke Ronald J C L M, van Leeuwen Paul A M, Meijer Sybren, Wijnands Pepijn G J T B, Statius Muller Markwin G, Busch Dirk H, Scheper Rik J, de Gruijl Tanja D
Abstract excerpt
Immune responses against tumor antigens will initially occur in the first tumor-draining lymph node, the sentinel node (SN). Because of extensive diagnostic procedures, obtaining a piece of SN to isolate viable immune cells for functional analyses is often impossible. For this reason an alternative method to obtain viable cells from a lymph node (LN) was investigated, ie, scraping LNs with a surgical blade, and...
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