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Article

Target-selected inactivation of the zebrafish rag1 gene.

Science (New York, N.Y.) - 5 Jul 2002

Wienholds Erno, Schulte-Merker Stefan, Walderich Brigitte, Plasterk Ronald H A

Abstract excerpt

The zebrafish has become a favorite organism for genetic analysis of vertebrate development, but methods for generating mutants by reverse genetic approaches have been lacking. We report a method to obtain stable mutants of a gene based on knowledge of the gene sequence only. Parental fish were mutagenized with N-ethyl-N-nitrosourea; in 2679 F1 fish, the rag1 gene was analyzed for heterozygous mutations by...

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PMID: 12098699
DOI: 10.1126/science.1071762

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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