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Article

In vitro selection of fibronectin gain-of-function mutations.

The Biochemical journal - 1 Jul 2002

Tani Patricia H, Loftus Joseph C, Bowditch Ron D

Abstract excerpt

Directed protein evolution, which employs a combination of random mutagenesis, phage display, and in vitro selection, was used to identify second-site suppressors of the fibronectin (Fn) cell binding domain mutation Asp1495Ala (RGA). The mutations in the Fn 9th (3fn9) and 10th (3fn10) type III repeats obtained after selection on purified integrins alphaIIbbeta3(D119Y) and alpha5beta1 are reported. The...

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Identifiers

PMID: 12071856
DOI: 10.1042/BJ20020067
PMCID: PMC1222673

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Source receipt: pubmed, retrieved 7 Sept 2026.

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