Article
In vitro selection of fibronectin gain-of-function mutations.
The Biochemical journal - 1 Jul 2002
Tani Patricia H, Loftus Joseph C, Bowditch Ron D
Abstract excerpt
Directed protein evolution, which employs a combination of random mutagenesis, phage display, and in vitro selection, was used to identify second-site suppressors of the fibronectin (Fn) cell binding domain mutation Asp1495Ala (RGA). The mutations in the Fn 9th (3fn9) and 10th (3fn10) type III repeats obtained after selection on purified integrins alphaIIbbeta3(D119Y) and alpha5beta1 are reported. The...
Topics
- Base Sequence
- Binding Sites
- DNA
- Directed Molecular Evolution
- Fibronectins
- Humans
- In Vitro Techniques
- Integrins
- Kinetics
- Ligands
- Models, Molecular
- Mutagenesis
- Mutation
- Peptide Library
- Protein Conformation
- Protein Engineering
- Recombinant Fusion Proteins