Article
Rapid genotyping of loci involved in antifolate drug resistance in Plasmodium falciparum by primer extension.
International journal for parasitology - 15 Jun 2002
Nair Shalini, Brockman Alan, Paiphun Lucy, Nosten François, Anderson Tim J C
Abstract excerpt
Current methods used to genotype point mutations in Plasmodium falciparum genes involved in resistance to antifolate drugs include restriction digestion of PCR products, allele-specific amplification or sequencing. Here we demonstrate that known point mutations in dihydrofolate reductase and dihydropteroate synthase can be scored quickly and accurately by single-nucleotide primer extension and detection of...
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