Article
Delineating the site of interaction on the pIII protein of filamentous bacteriophage fd with the F-pilus of Escherichia coli.
Journal of molecular biology - 7 Jun 2002
Deng Lih-Wen, Perham Richard N
Abstract excerpt
The minor coat protein pIII at one end of the filamentous bacteriophage fd, mediates the infection of Escherichia coli cells displaying an F-pilus. pIII has three domains (D1, D2 and D3), terminating with a short hydrophobic segment at the C-terminal end. Domain D2 binds to the tip of F-pilus, which is followed by retraction of the pilus and penetration of the E. coli cell membrane, the latter involving an...
Topics
- Bacteriophage M13
- Binding, Competitive
- Biotinylation
- Capsid Proteins
- DNA-Binding Proteins
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation
- Pili, Sex
- Protein Binding
- Protein Structure, Quaternary
- Protein Structure, Tertiary
- Subtilisin
- Viral Fusion Proteins
- Virion