Article
RNA polymerase II complexes in the very early phase of transcription are not susceptible to TFIIS-induced exonucleolytic cleavage.
Nucleic acids research - 1 Jun 2002
Sijbrandi Robert, Fiedler Ulrike, Timmers H Th Marc
Abstract excerpt
TFIIS is a transcription elongation factor for RNA polymerase II (pol II), which can suppress ribonucleotide misincorporation. We reconstituted transcription complexes in a highly purified pol II system on adenovirus Major-Late promoter constructs. We noted that these complexes have a high propensity for read-through upon GTP omission. Read-through occurred during the early stages at all registers analyzed....
Topics
- Adenoviridae
- Base Sequence
- DNA
- Humans
- Kinetics
- Macromolecular Substances
- Mutation
- Nucleic Acid Heteroduplexes
- Promoter Regions, Genetic
- RNA
- RNA Polymerase II