Article
Construction and phenotypic characterization of an auxotrophic mutant of Mycobacterium tuberculosis defective in L-arginine biosynthesis.
Infection and immunity - 1 Jun 2002
Gordhan Bhavna G, Smith Debbie A, Alderton Heidi, McAdam Ruth A, Bancroft Gregory J, Mizrahi Valerie
Abstract excerpt
A mutant of Mycobacterium tuberculosis defective in the metabolism of L-arginine was constructed by allelic exchange mutagenesis. The argF mutant strain required exogenous L-arginine for growth in vitro, and in the presence of 0.96 mM L-arginine, it achieved a growth rate and cell density in stationary phase comparable to those of the wild type. The mutant strain was also able to grow in the presence of high...
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