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Telomere measurement by quantitative PCR

15 May 2002

Abstract excerpt

It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR amplification with oligonucleotide primers designed to hybridize to the TTAGGG and CCCTAA repeats, because only primer dimer-derived products are expected. Here we present a primer pair that eliminates this problem, allowing simple and rapid measurement of telomeres in a closed tube, fluorescence-based assay. This assay will...

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PMID: 12000852
DOI: 10.1093/nar/30.10.e47

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