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Article

A quantitative PCR method for measuring absolute telomere length

31 Jan 2011

Abstract excerpt

We describe a simple and reproducible method to measure absolute telomere length (aTL) using quantitative real-time polymerase chain reaction (qPCR). This method is based on the Cawthon method for relative measurement of telomere length (TL) but modified by introducing an oligomer standard to measure aTL. The method describes the oligomer standards, the generation of the standard curve and the calculations...

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PMID: 21369534
DOI: 10.1186/1480-9222-13-3

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