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Article

Insertion of transposon Tn5tac1 in the Sinorhizobium meliloti malate dehydrogenase (mdh) gene results in conditional polar effects on downstream TCA cycle genes.

Molecular plant-microbe interactions : MPMI - 1 Dec 2004

Dymov Sergiy I, Meek David J J, Steven Blaire, Driscoll Brian T

Abstract excerpt

To isolate Sinorhizobium meliloti mutants deficient in malate dehydrogenase (MDH) activity, random transposon Tn5tac1 insertion mutants were screened for conditional lethal phenotypes on complex medium. Tn5tac1 has an outward-oriented isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoter (Ptac). The insertion in strain Rm30049 was mapped to the mdh gene, which was found to lie directly upstream of the...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 15597737
DOI: 10.1094/MPMI.2004.17.12.1318

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Source receipt: pubmed, retrieved 7 Sept 2026.

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