Article
Phenotypic effects of continuous or discontinuous treatment with dexamethasone and/or calcitriol on osteoblasts differentiated from rat bone marrow stromal cells.
Journal of cellular biochemistry - 1 Jan 2002
Atmani Hassan, Audrain Christine, Mercier Louis, Chappard Daniel, Basle Michel F
Abstract excerpt
Osteoblasts are target cells for glucocorticoids and calcitriol, and their phenotype is greatly modified by these hormones. We investigated the effect of continuous or discontinuous hormonal exposure to osteoblasts derived from rat bone marrow stromal cells in long-term subcultures. Stromal cells were grown in primoculture in presence of dexamethasone (dex), but in following subcultures, dex and/or calcitriol...
Topics
- Alkaline Phosphatase
- Animals
- Bone Marrow Cells
- Calcification, Physiologic
- Calcitriol
- Calcium
- Cell Differentiation
- Cell Division
- Cells, Cultured
- Dexamethasone
- Gene Expression
- Glucocorticoids
- Integrin-Binding Sialoprotein