Article
Isolation and characterization of tightly coupled mitochondria from wild type and nap mutant Neurospora crassa.
Biochemistry. Biokhimiia - 1 Feb 2002
Isakova E P, Gorpenko L V, Shurubor E I, Belozerskaya T A, Zvyagilskaya R A
Abstract excerpt
A fast and reproducible procedure was elaborated for isolation of tightly coupled mitochondria from wild type and nap-mutant Neurospora crassa cells harvested at different growth stages. The isolated mitochondrial preparations had controlled metabolic states and were tightly coupled, i.e., displayed good respiratory control and had close to the theoretically expected maximal ADP/O ratios upon oxidation of Krebs...
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