Panhandle and reverse-panhandle PCR enable cloning of der(11) and der(other) genomic breakpoint junctions of <i>MLL</i> translocations and identify complex translocation of <i>MLL</i> , <i>AF-4</i> , and <i>CDK6</i>

Abstract excerpt

We used panhandle PCR to clone the der(11) genomic breakpoint junction in three leukemias with t(4;11) and devised reverse-panhandle PCR to clone the breakpoint junction of the other derivative chromosome. This work contributes two elements to knowledge on MLL translocations. First is reverse-panhandle PCR for cloning breakpoint junctions of the other derivative chromosomes, sequences of which are germane to...

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