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Article

Panhandle PCR for cDNA: a rapid method for isolation of MLL fusion transcripts involving unknown partner genes.

Proceedings of the National Academy of Sciences of the United States of America - 15 Aug 2000

Megonigal M D, Rappaport E F, Wilson R B, Jones D H, Whitlock J A, Ortega J A, Slater D J, Nowell P C, Felix C A

Abstract excerpt

Identifying translocations of the MLL gene at chromosome band 11q23 is important for the characterization and treatment of leukemia. However, cytogenetic analysis does not always find the translocations and the many partner genes of MLL make molecular detection difficult. We developed cDNA panhandle PCR to identify der(11) transcripts regardless of the partner gene. By reverse transcribing first-strand cDNAs with...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 10920186
DOI: 10.1073/pnas.150241797
PMCID: PMC16910

Genes

ELL

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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Panhandle PCR for cDNA: a rapid method for isolation of MLL fusion transcripts involving unknown partner genes.PMID 10920186Read ↗

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