Article
Solution NMR spectroscopy of [alpha -15N]lysine-labeled rhodopsin: The single peak observed in both conventional and TROSY-type HSQC spectra is ascribed to Lys-339 in the carboxyl-terminal peptide sequence.
Proceedings of the National Academy of Sciences of the United States of America - 19 Mar 2002
Klein-Seetharaman J, Reeves P J, Loewen M C, Getmanova E V, Chung J, Schwalbe H, Wright P E, Khorana H G
Abstract excerpt
[alpha-(15)N]Lysine-labeled rhodopsin, prepared by expression of a synthetic gene in HEK293 cells, was investigated both by conventional and transverse relaxation optimized spectroscopy-type heteronuclear single quantum correlation spectroscopy. Whereas rhodopsin contains 11 lysines, 8 in cytoplasmic loops and 1 each in the C-terminal peptide sequence and the intradiscal and transmembrane domains, only a single...
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