Article
Solution 19F nuclear Overhauser effects in structural studies of the cytoplasmic domain of mammalian rhodopsin.
Proceedings of the National Academy of Sciences of the United States of America - 24 Apr 2001
Loewen M C, Klein-Seetharaman J, Getmanova E V, Reeves P J, Schwalbe H, Khorana H G
Abstract excerpt
19F nuclear Overhauser effects (NOEs) between fluorine labels on the cytoplasmic domain of rhodopsin solubilized in detergent micelles are reported. Previously, high-resolution solution (19)F NMR spectra of fluorine-labeled rhodopsin in detergent micelles were described, demonstrating the applicability of this technique to studies of tertiary structure in the cytoplasmic domain. To quantitate tertiary contacts we...
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