Article
Abnormal accumulation of hyaluronan matrix diminishes contact inhibition of cell growth and promotes cell migration.
Proceedings of the National Academy of Sciences of the United States of America - 19 Mar 2002
Itano Naoki, Atsumi Fukiko, Sawai Takahiro, Yamada Yoichi, Miyaishi Osamu, Senga Takeshi, Hamaguchi Michinari, Kimata Koji
Abstract excerpt
Elevated hyaluronan biosynthesis and matrix deposition correlates with cell proliferation and migration. We ectopically expressed three isoforms of hyaluronan synthase (HAS1, HAS2, or HAS3) in nontransformed rat 3Y1 cells and observed a de novo, massive formation of a hyaluronan matrix that resulted in a partial loss of contact-mediated inhibition of cell growth and migration. All three HAS transfectants showed...
Topics
- Agar
- Animals
- Cell Count
- Cell Division
- Cell Line
- Cell Movement
- Cell Size
- Cell Transformation, Neoplastic
- Contact Inhibition
- Cytoskeleton