Article
Effect of different concentrations of H-NS protein on chromosome replication and the cell cycle in Escherichia coli.
Journal of bacteriology - 1 Apr 2002
Atlung T, Hansen F G
Abstract excerpt
Flow cytometric analysis showed that the hns205 and hns206 mutants, lacking the abundant nucleoid-associated protein H-NS, have decreased origin concentration, as well as a low number of origins per cell (ploidy). The most striking observation was that the low ploidy was due to a very short replication time, e.g., at 30 degrees C it was halved compared to that of the hns(+) strain. The decreased origin...
Topics
- Bacterial Proteins
- Binding Sites
- Cell Cycle
- Chromosomes, Bacterial
- DNA Replication
- DNA-Binding Proteins
- Dose-Response Relationship, Drug
- Electrophoretic Mobility Shift Assay
- Escherichia coli
- Escherichia coli Proteins
