Article
Molecular analysis of the Escherichia coli hns gene encoding a DNA-binding protein, which preferentially recognizes curved DNA sequences.
Molecular & general genetics : MGG - 1 Nov 1991
Yamada H, Yoshida T, Tanaka K, Sasakawa C, Mizuno T
Abstract excerpt
We previously demonstrated that the E. coli protein, H-NS (or H1a), encoded by the gene hns (or osmZ or bglY) preferentially recognizes curved DNA sequences in vitro. In order to gain further insight into the complex function of H-NS and the significance of DNA curvature, we constructed a structurally defined hns deletion mutant on the E. coli chromosome. The hns deletion mutant thus obtained showed a variety of...
Topics
- Bacterial Proteins
- Chromosome Deletion
- Chromosomes, Bacterial
- DNA, Bacterial
- DNA-Binding Proteins
- Electrophoresis, Gel, Two-Dimensional
- Escherichia coli
- Genes, Bacterial
- Mutation
- Nucleic Acid Conformation