Article
Enzymatic modification of tRNAs: MiaB is an iron-sulfur protein.
The Journal of biological chemistry - 19 Apr 2002
Pierrel Fabien, Björk Glenn R, Fontecave Marc, Atta Mohamed
Abstract excerpt
The product of the miaB gene, MiaB, from Escherichia coli participates in the methylthiolation of the adenosine 37 residue during modification of tRNAs that read codons beginning with uridine. A His-tagged version of MiaB has been overproduced and purified to homogeneity. Gel electrophoresis and size exclusion chromatography revealed that MiaB protein is a monomer. As isolated MiaB contains both iron and sulfide...
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